目的建立高效液相色谱(HPLC)法同时测定中药制剂烧伤膏中5个蒽醌类化合物(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚)的含量。方法以5个蒽醌类成分为指标,选择Diamonsil C18(4.6 mm×250 mm,5 μm)色谱柱,使用甲醇-乙腈0.1%磷酸(23∶42∶35)为流动相进行洗脱。结果5个蒽醌类成分分别在0.005 5～0.176 1 μg(芦荟大黄素r=0.999 9)、0.010 1～0.323 7 μg(大黄酸r=0.999 9)、0.016 6～0.522 3 μg(大黄素r=0.999 9)、0.0216～0.690 6 μg(大黄酚r=0.999 9)和0.030 3～0.968 8 μg(大黄素甲醚r=0.999 9)内,与峰面积呈良好的线性关系;平均回收率分别为97.6%、96.6%、98.8%、99.2%、97.5%;RSD(n=6)分别为2.0%、1.8%、2.9%、1.3%、1.2%。结论该方法简便、可靠、重复性好,适用于烧伤膏中大黄的质量控制。
Abstract
OBJECTIVE To establish a method for determining the contents of aloe-emodin, rhein, emodin, chrysophanol and physcion in the burn ointment. METHODS Diamonsil C18 column (4.6 mm×250 mm,5 μm) was adopted, with methanol-acetonitrile-0.1% phosphoric acid (23∶42∶35) as the mobile phase. The detection wavelength was set at 254 nm, the flow rate was 1 mL·min-1, and the column temperature was 30 ℃.RESULTS The liner rangers of aloe-emodin, rhein, emodin, chrysophanol and physcion were within 0.005 5-0.176 1 μg (r=0.999 9), 0.010 1-0.323 7 μg (r=0.999 9), 0.016 6-0.522 3 μg (r=0.999 9), 0.021 6-0.690 6 μg (r=0.999 9) and 0.030 3-0.968 8 μg (r=0.999 9), respectively. The average recoveries were 97.6% (RSD=2.0%),96.6% (RSD=1.8%), 98.8% (RSD=2.9%), 99.2% (RSD=1.3%), and 97.5% (RSD=1.2%), respectively (n=6). CONCLUSION This method is specific, accurate, reproducible, and can be used for the determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in the burn ointment.
关键词
烧伤膏 /
蒽醌 /
芦荟大黄素 /
高效液相色谱法 /
含量测定
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Key words
burn ointment /
anthraquinone /
aloe-emodin /
HPLC /
content determination
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中图分类号：
R917
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参考文献
[1] YUAN T. Researches about topical TCM in the treatment of burns. Guangming J Chin Med(光明中医), 2010, 25(11):2151- 2153. [2] LI J Z, XIAY, LIU B D, et al. The promotion mechanism of aloe-emodin on skin wound healing. Chin J Basic Med Tradit Chin Med (中国中医基础基础医学杂志), 2011, 17(11):1260-1263. [3] ZUO Y J, LIAO Q W, WANG Y H. Progress on drug research for treating burn trauma. Hunan Guiding J Tradit Chin Med Pharmacol(湖南中医药导报), 2012, 3(8):106-108. [4] Ch. P (2010) VolⅠ(中国药典2010版. 一部). 2010:20. [5] WANG L, ZHANG J W, GAO W Y. Pharmacokinetic researches about the active ingredient of rhubarb. Chin Tradit Patent Med (中成药), 2011, 33(9):1571-1574. [6] HE F Z, CAI X Y, PENG W, et al. Simultaneous determination of five anthraquinone compounds in rhubarb by HPLC. J Chin Med Mater (中药材), 2011,34(9):1384-1385. [7] NING J. Determination of emodin and chrysophanol in anti-vertigo particle by HPLC. Chin Tradit Herbal Drugs (中草药), 2010,5(41):749-950. [8] GAO X Y, JIANG Y, LU J Q, et al. One single standard substance for the determination of multiple anthraquinone derivatives in rhubarb using high-performance liquid chromatography-diode array detection. J Chromatogr A, 2009,12(16):2118-2123. [9] YANG Q F, PENG L B, LI J, et al. Determinationof chrysophanol, liquiritin and naringin in shenfushu granules by HPLC-DAD. Chin Phram J (中国药学杂志), 2011,16(46):1283-1285. [10] GAO X Y, LU J Q. HPLC-DAD Simultaneous determination of 7 anthraquinone derivatives in Rhubarb. Chin J Pharm Anal (药物分析杂志), 2010, 30(9):1636-1641. [11] XU Q L, MAO X T, et al. HPLC Simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physic on in Liuwei Anxiao capsules. Chin J Pharm Anal (药物分析杂志), 2010, 30(10):1841-1843. [12] KUANG X H, DONG X P, SHI R B, et al. Chinese Chemical (中药化学). 2th ed. Beijing:China Press of Traditional Chinese Medicine, 2010:71. [13] CHEN X T, HUANG J H. Determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Daochi pills by HPLC. Guide China Med(中国医药指南), 2011, 27(9):179-180.
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脚注
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基金
国家自然科学基金面上资助项目(81173632);安徽省卫生厅中医药科研资助项目(2012zy52);2013年安徽省省级中医药发展专项基金资助项目;安徽省高等教育振兴计划人才项目省学术带头人培养资助项目(2013)
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